Table 1.
Micronucleus assays results. Bone marrow cytotoxicity expressed as polychromatic erythrocytes (PCE) out of total erythrocytes (normochromatic erythrocytes (NCE) + PCE), ratio of PCE out of NCE, and the micronuclei induction expressed as % MN-PCE´s. The values are expressed as mean ± SD. Significantly different from the negative and solvent control (** p < 0.01).
| Groups | Sex | n | Doses | PCE/Total | % MN-PCE´s | PCE/NCE |
|---|---|---|---|---|---|---|
| Negative Control (water) |
♂ | 5 | 0.49 ± 0.02 | 0.45 ± 0.64 | 0.95 ± 0.08 | |
| ♀ | 5 | 0.49 ± 0.03 | 0.66 ± 0.32 | 0.96 ± 0.14 | ||
| Solvent Control (0.5% MeOH) |
♂ | 5 | 0.50 ± 0.02 | 0.83 ± 0.51 | 0.98 ± 0.08 | |
| ♀ | 5 | 0.50 ± 0.03 | 0.58 ± 0.24 | 1.00 ± 0.11 | ||
| Positive Control (EMS *) | ♂ | 3 | 200 mg/kg b.w. | 0.34 ± 0.02 ** | 1.93 ± 0.15 ** | 0.51 ± 0.05 ** |
| ♀ | 3 | 0.36 ± 0.03 ** | 2.47 ± 0.9 ** | 0.57 ± 0.08 ** | ||
| CYN/MC-LR | ♂ | 5 | 7.5 + 75 µg/kg b.w. | 0.51 ± 0.02 | 1.75 ± 0.42 ** | 1.03 ± 0.07 |
| ♀ | 5 | 0.50 ± 0.03 | 1.98 ± 0.21 ** | 1.05 ± 0.13 | ||
| ♂ | 5 | 23.7 + 237 µg/kg b.w. | 0.46 ± 0.04 | 1.83 ± 0.41 ** | 0.86 ± 0.14 | |
| ♀ | 5 | 0.47 ± 0.03 | 2.09 ± 0.15 ** | 0.88 ± 0.09 | ||
| ♂ | 5 | 75 + 750 µg/kg b.w. | 0.37 ± 0.03 ** | 1.88 ± 0.51 ** | 0.60 ± 0.08 ** | |
| ♀ | 5 | 0.34 ± 0.06 ** | 2.22 ± 0.27 ** | 0.51 ± 0.12 ** |
* EMS: ethylmethanesulfonate.