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General steps of the NGS workflow for HIVDR genotyping. Common steps for most of the second-generation NGS workflows are shown, including the main sources of variation and possible bias associated with each step. NGS—next generation sequencing; PBMC—peripheral blood mononuclear cells; DBS—dried blood spots; PCR—polymerase chain reaction; QC—quality control; QA—quality assurance; HIVDR—HIV drug resistance.
