Table 2.
Autologous and nonautologous patterns of core NEC protein nuclear rim colocalization: selected examples derived from α-, β- and γ-Herpesvirinae. Various combinations of NEC interactions were analyzed on the basis of pairwise transient cotransfection of NEC-encoding expression plasmids, i.e., α-herpesviral pUL34/Orf24, pUL31/Orf27 (HSV-1/VZV); β-herpesviral pUL50/pM50, pUL53/pM53 (HCMV/MCMV); γ-herpesviral BFRF1/Orf67, BFLF2/Orf69 (EBV/ KSHV). Confocal microscopic evaluation of rim-like nuclear envelope colocalization of autologous/ nonautologous core NEC pairs, transiently coexpressed in HeLa cells. Localization phenotypes: -, colocalization in < 5% of cells; ±, 5%-25%; +, 25.1%-75%; ++, >75%.
| Hook Proteins | ||||||||
|---|---|---|---|---|---|---|---|---|
| alpha | beta | gamma | ||||||
| HSV-1 pUL31 |
VZV Orf27 |
HCMV pUL53 |
MCMV pM53 |
EBV BFLF2 |
KSHV Orf69 |
|||
| Groove Proteins | alpha | HSV-1 pUL34 |
++ | + | - | - | - | - |
| VZV Orf24 |
+ | ++ | - | - | - | - | ||
| beta | HCMV pUL50 |
- | - | ++ | ++ | - | - | |
| MCMV pM50 |
- | - | + | ++ | - | - | ||
| gamma | EBV BFRF1 |
- | - | - | - | ++ | + | |
| KSHV Orf67 |
- | - | - | - | + | ++ | ||