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. 2020 Jun 4;12(6):373. doi: 10.3390/toxins12060373

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cytokine-driven amplification of ex vivo cultured CD34⁺ HSPCs monitored in a time interval of 15 days (A) and the portrayal of cell surface markers of the developing erythrocytes (B). Cells were cultivated in the presence of interleukin (IL)-3, stem cell factor (SCF), and erythropoietin (EPO), which were applied from the initiation of cultivation at day 0 until day 8 (IL-3), day 11 (SCF), and the end of cultivation at day 15 (EPO). The graph shows the average cell expansion of HSPCs of three donors ([145], modified).