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. 2020 Jun 23;12(6):678. doi: 10.3390/v12060678

Table 1.

Primers tested to isolate the kcv genes.

Name Sequence Position GC Content (%) Tm (°C)
Forward Primers Kcv6 Frw CTT TAG YYT TYY TCK GVC −366 34 49
Kcv7 Frw CTT TAG YYT TYY TCK GVC G −366 38 54
Kcv8 Frw GAA GCA GGY ACC ACT TTA G −379 47 53
Kcv9 Frw GCA GGY ACC ACT TTA G −376 50 47
Reverse Primers Kcv6 Rvs CRC RGM ATR TRT CAT TTG WCC C +256 48 64
Kcv7 Rvs CTT ACR CRG MAT RTR TCA TTT G +259 39 56
Kcv8 Rvs CTT ACR CRG MAT RTR TC +264 44 44
Kcv9 Rvs HKB YMC GAT CTT ATA CAC +292 39 45
Kcv10 Rvs CAT TTC TTA CRC RGM ATR TRT C +264 39 55