Table 1.
Name | Sequence | Position | GC Content (%) | Tm (°C) | |
---|---|---|---|---|---|
Forward Primers | Kcv6 Frw | CTT TAG YYT TYY TCK GVC | −366 | 34 | 49 |
Kcv7 Frw | CTT TAG YYT TYY TCK GVC G | −366 | 38 | 54 | |
Kcv8 Frw | GAA GCA GGY ACC ACT TTA G | −379 | 47 | 53 | |
Kcv9 Frw | GCA GGY ACC ACT TTA G | −376 | 50 | 47 | |
Reverse Primers | Kcv6 Rvs | CRC RGM ATR TRT CAT TTG WCC C | +256 | 48 | 64 |
Kcv7 Rvs | CTT ACR CRG MAT RTR TCA TTT G | +259 | 39 | 56 | |
Kcv8 Rvs | CTT ACR CRG MAT RTR TC | +264 | 44 | 44 | |
Kcv9 Rvs | HKB YMC GAT CTT ATA CAC | +292 | 39 | 45 | |
Kcv10 Rvs | CAT TTC TTA CRC RGM ATR TRT C | +264 | 39 | 55 |