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. 2020 Jun 19;12(6):665. doi: 10.3390/v12060665

Figure 6.

Figure 6

Presence of increased RNA Pol II and p300 on HIV-1 genome. CEM EVs were isolated using ultracentrifugation. ACH2 (A) and U1 (B) cells were then plated and treated with EVs at 1:5000. Cells were incubated for 24 h and again treated with CEM EVs at 1:5000. Following a second 24-h incubation, cells were harvested and crosslinked prior to ChIP assay using antibodies against IgG, Pol II, p65, and p300. DNA was then quantified using PCR using NF-κB1-2F and TAR +59-R. IgG was used as a background control. A Student’s T-test was used to compare untreated control and EV-treated samples. *, p < 0.05; **, p < 0.01; ***, p < 0.001.