Figure 6.

Human norovirus in stool samples from chronic shedders is infectious in human intestinal enteroids. 2D fetal ileum enteroids were differentiated for 6 days and then infected with unfiltered human norovirus-positive stools for 3 days. Viral genome copy numbers were measured at 0 and 3 dpi by RT-qPCR. Each sample was tested in triplicate and the entire experiment repeated twice. The fold-increase in viral titer was determined by comparing genome titers at 3 to 0 dpi. Replication data in HEK293T cells for each stool sample tested in enteroids are shown for comparison. The dashed line represents the lower limit of detection. Values for technical replicates were averaged and error bars indicate the mean of experimental repeats.