Figure 5.

Effect of PRMT5 knockdown on BLV protein expression. FLK-BLV (A) or PK15-BLV (B) cells were transfected with scramble siRNA (si-NC) or siRNA targeting PRMT5 (si-PRMT5) at the indicated concentrations for 48 h for FLK-BLV or 72 h for PK15-BLV. Cell lysates were prepared and the expression of two viral proteins (Env gp51 and Gag p24) were assessed by Western blotting. Knockdown efficiency was evaluated by evaluating PRMT5 protein expression by Western blotting. The relative band density of each protein normalized to α-tubulin expression is shown for each cell line. Error bars represent the standard deviation of three experiments. The p value was calculated by Student’s t-test. The asterisk indicates a significant difference (* p ≤ 0.05, and *** p ≤ 0.001). Positions of BLV gp51 and p24, PRMT5, and α-tubulin proteins are indicated.