Table 2.
Oligonucleotides.
| Name | Sequence (5′–3′) | Supplier | Use |
|---|---|---|---|
| HBV_RC_F | CACTCTATGGAAGGCGGGTA | Eurofins Genomics | qPCR for quantification of HBV stocks |
| HBV_RC_R | TGCTCCAGCTCCTACCTTGT | Eurofins Genomics | qPCR for quantification of HBV stocks |
| HBV RNA 3 F | GCTTTCACTTTCTCGCCAAC | Eurofins Genomics | RT-qPCR |
| HBV RNA 3 R | GAGTTCCGCAGTATGGATCG | Eurofins Genomics | RT-qPCR |
| ISG54_F | GGTGGCAGAAGAGGAAGATT | Eurofins Genomics | RT-qPCR |
| ISG54_R | TAGGCCAGTAGGTTGCACAT | Eurofins Genomics | RT-qPCR |
| IFNλ-1_F | CGCCTTGGAAGAGTCACTCA | Eurofins Genomics | RT-qPCR |
| IFNλ-1_R | GAAGCCTCAGGTCCCAATTC | Eurofins Genomics | RT-qPCR |
| cGAS_F | CAAGAAGGCCTGCGCATTCA | Eurofins Genomics | RT-qPCR |
| cGAS_R | GAGAAGGATAGCCGCCATGT | Eurofins Genomics | RT-qPCR |
| STING_F | GATATCTGCGGCTGATCCTG | Eurofins Genomics | RT-qPCR |
| STING_R | GCTGTAAACCCGATCCTTGA | Eurofins Genomics | RT-qPCR |
| IFI16_F | CGCTTGAAGACCTGGCTGAA | Eurofins Genomics | RT-qPCR |
| IFI16_R | TGACAGTGCTGCTTGTGGAG | Eurofins Genomics | RT-qPCR |
| PQBP1_F | TCTGGAGCCTGAACCAGAGGAA | Eurofins Genomics | RT-qPCR |
| PQBP1_R | TCCAACCTGGTGGCCTCGTAGT | Eurofins Genomics | RT-qPCR |
| RIG-I_F | CCTACCTACATCCTGAGCTACAT | Eurofins Genomics | RT-qPCR |
| RIG-I_R | TCTAGGGCATCCAAAAAGCCA | Eurofins Genomics | RT-qPCR |
| MAVS_F | GGTGCTCACCAAGGTGTCTG | Eurofins Genomics | RT-qPCR |
| MAVS_R | AGGAGGTGCTGGCACTGATG | Eurofins Genomics | RT-qPCR |
| MDA5_F | AGAGTGGCTGTTTACATTGCC | Eurofins Genomics | RT-qPCR |
| MDA5_R | GCTGTTCAACTAGCAGTACCTT | Eurofins Genomics | RT-qPCR |
| TLR3_F | ACCTCCAGCACAATGAGCTA | Eurofins Genomics | RT-qPCR |
| TLR3_R | TCCAGCTGAACCTGAGTTCC | Eurofins Genomics | RT-qPCR |
| RPL13A_F | CCT GGA GGA GAA GAG GAA AGA GA | Eurofins Genomics | RT-qPCR |
| RPL13A_R | TTG AGG ACC TCT GTG TAT TTG TCA A | Eurofins Genomics | RT-qPCR |
| TBP_F | GGAGCTGTGATGTGAAGT | Eurofins Genomics | RT-qPCR |
| TBP_R | TACGTCITCTTCCTGAATCC | Eurofins Genomics | RT-qPCR |
| HBV_quant_F | TGTCAACACTAATATGGGCCTAA | Eurofins Genomics | quantification of HBV RNA and DNA |
| HBV_quant_R | AGGGGCATTTGGTGGTCTAT | Eurofins Genomics | quantification of HBV RNA and DNA |
| HBV_st | TGTCAACACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGAGAAACAGTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGCTTATAGACCACCAAATGCCCCT | Integrated DNA Technologies, BvBA | standard for quantification of HBV RNA and DNA |
| MVAgfp_F | AGCACGACTTCTTCAAGTCC | Eurofins Genomics | quantification of MVAgfp DNA |
| MVAgfp_R | GTTGTAGTTGTACTCCAGCTTG | Eurofins Genomics | quantification of MVAgfp DNA |
| MVAgfp_st | AGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAAC | Integrated DNA Technologies, BvBA | standard for quantification of MVAgfp DNA |
| SeV_F | TTCATTATCATCCCGTGAGA | Eurofins Genomics | quantification of SeV RNA |
| SeV_R | CCAGTGATCCATCATCAATC | Eurofins Genomics | quantification of SeV RNA |
| SeV_st | TTCATTATCATCCCGTGAGATCAGGAACCTGAGGGTTATCACAAAAACTTTATTAGACAGGTTTGAGGATATTATACATAGTATAACGTATAGATTCCTCACCAAAGAGATAAAGATTTTGATGAAGATTTTAGGGGCAGTCAAGATGTTCGGGGCCAGGCAAAATGAATACACGACCGTGATTGATGATGGATCACTGG | Integrated DNA Technologies, BvBA | standard for quantification of SeV RNA |
(Eurofins Genomics, Ebersberg, Germany; Integrated DNA Technologies, BvBA, Coralville, IA, USA).