Table 1.

Chemical identification of the components of RIPS fractions by high resolution mass spectrometry, in positive and negative electrospray ionization modes (ESI(+) and ESI(−), respectively).

Entry	Precursor Ion m/z	Extract	Identification	Elem. Comp.	Diff ppm	Comp. Type	Ref.
Extract analysis in positive mode ESI(+)
1	191.1171	RI24	N-Methyl-5-hydroxytryptamine	C11H14N2O	7.00	Alkaloid	[40,41,42,43]
2	391.2497	RI23	Sarmentogenin	C23H34O5	3.20	Steroid	[44]
3	399.2189	RI18	(5β,12β)-12,14-Dihydroxy-11-oxobufa-3,20,22-trienolide	C24H30O5	4.39	Steroid	[45,46]
	399.2182	RI23			2.63
4	401.2306	RI18	14,15-Epoxy-3,5-dihydroxybufa-20,22-dienolide (marinobufagin)	C24H32O5	5.48	Steroid	[25,31,47,48]
	401.2318	RI23			2.50
5	403.2446	RI23	(3β,5β,16β)-3,5,16-Trihydroxy-bufa-14,20,22-dienolide (Bufogenin B)	C24H34O5	9.55	Steroid	[49,50]
6	435.2356	RI19	11α,19-Di-hydroxy-telocinobufagin	C24H34O7	6.15	Steroid	[46,51,52]
	433.2266	RI24			9.17
7	440.2167	RI23	Argentinogenin	C26H31O6	7.24	Steroid	[44,53]
8	445.2609	RI18	Bufotalin	C26H36O6	4.24	Steroid	[35,44,54,55,56,57]
9	458.2310	RI23	19-Oxo-cinobufagin	C26H32O7	1.19	Steroid	[36,44]
10	461.2481	RI24	Monohydroxylbufotalin	C26H36O7	8.73	Steroid	[6]
11	531.2983	RI23	3α,12β,25,26-Tetrahydroxy-7-oxo-5β-cholestane-26-O-sulfate	C27H46O8S	1.63	Steroid	[44]
12	599.3231	RI23	Cinobufagin-3-hemisuberate	C34H46O9	1.82	Steroid	[58]
Extract analysis in negative mode ESI(−)
13	433.2266	RI24	11α,19-Di-hydroxy-telocinobufagin (Marinosin)	C24H34O7	9.17	Steroid	[46,52,53]
14	431.2116	RI19	Marinoic acid (3β-hydroxy-11,12-seco-5β,14β-bufa-20,22-dienolide-11,14-olide-12-oic acid)	C24H30O7	3.43	Steroid	[59,60]

Comp. type–component type (broad classification). Elem. comp.—elemental composition. RI–Rhinella icterica.