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. 2020 May 30;12(6):594. doi: 10.3390/v12060594

Figure 1.

Figure 1

UL33 facilitates Human cytomegalovirus (HCMV) Merlin virus growth in vitro. (a) Schematic representation of the recombination strategy to construct HCMV Merlin mutant BACs. Designations of the BAC variations are shown on the left. TR, terminal repeat; IR, internal repeat; U, Unique region; L, long; S, short; (L), loxP sites; BAC, pBeloBAC11; Cre, Cre recombinase; (b) micrographs of HCMV virus growth in HFFF TR cells upon reconstitution of the different HCMV Merlin mutants via transfection of the cells with BAC DNA; micrograph size: 2.2 mm × 1.64 mm; (c) multistep growth kinetics of Merlin-UL33-HA and mutant viruses, as assessed by the number of secreted virus particles. Results are representative of 3 individual experiments and depicted as mean and SD.