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. 2020 Jun 22;12(6):670. doi: 10.3390/v12060670

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Infecting genome transit to the inner membrane and growth characteristics of the compensatory mutants. (A) Infecting genome transit to the inner membrane. Data are presented as I:O ratios, which is the genome copy number of the inner membrane over that of the outer membrane. I:O ratios of particles carrying wild-type H (black), M251Q H (blue), and T244Q/M251Q H (cyan) are shown. Error bars represent the standard deviation obtained from three technical replicates. (B) Growth characteristics of second site suppressors in an otherwise wild-type background. Titers of progeny produced in lysis-resistant cells infected with wild-type ϕX174 (black), su(H)-F T204I (dark green), or su(H)-F M330I (light green). Data are displayed as the viral burst size, which is calculated by dividing the total progeny by the total input phage. Experiments were conducted at MOIs less than 1.0 in lysis-resistant cells. Error bars depict the standard deviation obtained from three biological replicates.