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. 2020 Jun 11;12(6):387. doi: 10.3390/toxins12060387

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The methylation levels of DNA and enzyme activity of methyltransferase (DNMT) measured by enzyme-linked immunosorbent assay (ELISA). (A) The methylation levels of DNA. (B) The enzyme activity of DNMT. Cells were collected 24 h after the stimulation to isolate DNA and nucleoprotein for ELISA. The data are presented as mean ± standard deviation (SD). The error bars represent standard deviation, and the asterisk indicates statistical difference (n = 6, * p < 0.05) between the indicated columns, based on one-way analysis of variance followed by Duncan’s multiple comparison test. CON, control group; LPS, lipopolysaccharide group; PLL, the group of co-stimulation with peptidoglycan (PGN), lipoteichoic acid (LTA), and LPS.