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. 2020 Jun 25;12(6):684. doi: 10.3390/v12060684

Figure 1.

Figure 1

Isolation of scFv clones specific to human PD-L1s. (A) The individual specific binders are screened by ELISA and 72 different scFv clones were further characterized by sequencing. Reactivity of the selected scFv clones to human PD-L1 was assayed by measuring absorbance at 450 nm. The individual scFv clones were reactive to hPD-L1-Fc (■), but not hFc (□) or BSA (▨); (B) Binding activity of scFv (top 20 clones) on hPD-L1 293T cells measured using flow cytometry. 293T cells were transfected with human PD-L1s. Binding of periplasmic scFvs was detected by adding first anti-c-Myc murine monoclonal antibody followed by Alexa Flour 488-conjugated antimurine IgG (H+L).