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. 2020 Jun 25;12(6):684. doi: 10.3390/v12060684

Figure 7.

Figure 7

The application of the BLI-based assay for epitope characterization of antibodies. (A) Classification and assessment of abnormal binding sensorgrams in BLI-based PD-1/PD-L1-binding inhibition assays. In the assay, PD-1 is coated on the sensor and a mixture of PD-L1 and each IgG antibody clone was incubated with the sensor; (B) Distribution of #41 (‒144%) and #71 (‒37.63%) clones in BLI-based assays using IgG; (C) Binding compatibility test using the BLI assay. The PD-L1-coated sensors were pre-saturated with MPDL3280A antibody binding and two antibodies were introduced to the sensors consecutively to check if any clone has compatible binding with MPDL3280A (tested clones: #3, 6, 10, 24, 26, 29, 35, 40, 41, 50, 51, 52, 56, 63, 70, 71).