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. 2020 Jun 22;12(6):672. doi: 10.3390/v12060672

Figure 3.

Figure 3

FKBP5 interacts with IKKα. (A) Map of the FKBP5 protein interaction network. FKBP5 and the high confidence candidate interacting proteins (HCIPs) are shown as square and circles, respectively. (B) Vector or FLAG-tagged FKBP5 was co-transfected with Myc-tagged IKKα or IKKβ into HEK293 cells. After 48 h, cell lysates were immunoprecipitated and blotted as indicated. (C) HEK 293 cells were stimulated with 1 µg/mL poly(I:C) for 4 h. Cell lysates were immunoprecipitated and blotted as indicated. The same isotype Immunoglobulin G (IgG) was used as a control for immunoprecipitation (IP). (D) Schematics of the FKBP5 mutants. FK stands for FKBP; TPR stands for tetratricopeptide repeats. (E) Hemagglutinin (HA)-tagged IKKα was transfected with the pCMV-3Tag-8 vector, FLAG-tagged FKBP5, or the indicated FLAG-tagged FKBP5 mutant. After 48 h, the cell lysates were immunoprecipitated and blotted as indicated.