Figure 2.

The effect of the zearalenone hydrolase ZenA enzyme, added to the feed, on the zearalenone (ZEN) concentration in the in vitro rumen model. The enzyme was tested at two different concentrations (125 µg/mg feed and 62.5 µg/mg feed) during an incubation period of 48 h. The ZEN concentration is expressed relative to the maximum ZEN concentration detected. As the enzyme degraded ZEN very effectively, its concentration was below the detection limit from the first incubation timepoint and for both enzyme concentrations, resulting in overlapping results of the treatments 62.5 and 125 µg/mg.