Table 8.

Primers sequences and recycling conditions of PCR assays used for the detection of virulence genes and antimicrobial resistance genes.

Target Gene	Oligonucleotide Sequence (5′-3′)	Product Size (bp)	Denat.	Cycling (35)			Final E	References
				D	A	E
eaeA	F: GTGGCGAATACTGGCGAGACT R: CCCCATTCTTTTTCACCGTCG	248	95 °C 5 min	95 °C 30 s	60 °C 30 s	72 °C 30 s	72 °C 10 min	[41]
stx1	F: ACACTGGATGATCTCAGTGG R: CTGAATCCCCCTCCATTATG	614	95 °C 5 min	95 °C 3 min	59 °C 45 s	72 °C 90 s	72 °C 10 min	[42]
stx2	F: CCATGACAACGGACAGCAGTT R: CCTGTCAACTGAGCAGCACTTTG	779	95 °C 5 min	95 °C 3 min	59 °C 45 s	72 °C 90 s	72 °C 10 min	[42]
sta	F: GAAACAACATGACGGGAGGT R: GCACAGGCAGGATTACAACA	229	94 °C 5 min	94 °C 30 s	57 °C 45 s	72 °C 45 s	72 °C 10 min	[43]
lt	F: GGTTTCTGCGTTAGGTGGAA R: GGGACTTCGACCTGAAATGT	605	94 °C 5 min	94 °C 30 s	57 °C 45 s	72 °C 45 s	72 °C 10 min	[43]
f41	F: GCATCAGCGGCAGTATCT R: GTCCCTAGCTCAGTATTATCACCT	380	94 °C 5 min	94 °C 30 s	50 °C 45 s	72 °C 90 s	72 °C 10 min	[44]
aadB	F: GAGCGAAATCTGCCGCTCTGG R: CTGTTACAACGGACTGGCCGC	319	94 °C 5 min	94 °C 30 s	55 °C 45 s	72 °C 45 s	72 °C 10 min	[45]
blaTEM	F: ATCAGCAATAAACCAGC R: CCCCGAAGAAC GTTTTC	516	94 °C 5 min	94 °C 30 s	55 °C 45 s	72 °C 45 s	72 °C 10 min	[46]
sul1	F: CGGCGTGGGCTACCTGAACG R: GCCGATCGCGTGAAGTTCCG	433	94 °C 5 min	94 °C 30 s	55 °C 45 s	72 °C 45 s	72 °C 10 min	[47]

D, Denaturation; A, Annealing; E, Extension.