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. 2020 Jun 6;12(6):375. doi: 10.3390/toxins12060375

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Ligand blot analysis of His-tagged toxin-binding regions (TBRs) binding to activated Cry1Ac, Cry1Ab, or Cry1Fa. The His-tagged TBRs of HaCad, SlCad, SeCad, and SlCad were used at different concentrations (0.125, 0.250, and 0.50 μg/mL) with one lane for each His-TBR. Binding of all toxins was assayed at 10 nM. Bound toxin was revealed with the corresponding polyclonal antibody (rabbit anti-Cry1Ac, rabbit anti-Cry1Ab, or rabbit anti-Cry1Fa antibody) as indicated in the Materials and Methods Section.