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. 2020 Jun 3;12(6):610. doi: 10.3390/v12060610

Figure 4.

Figure 4

Relative expression of mRNA for tricarboxylic acid cycle genes in 13S and 12S expressing A549 cells. (A–O) The mRNA expression for 15 genes encoding components of the tricarboxylic acid (TCA) cycle were measured with qPCR. A549-13S cells displayed upregulated mRNA levels of enzymes involved in the TCA cycle. A549-13S cells had statistically significant upregulation of transcripts encoding six enzymes involved in the TCA cycle (B,G,J,K,M,N) and downregulation of two TCA cycle enzyme encoding transcripts (F,I). In contrast, A549-12S cells did not exhibit any statistically significant differences in TCA cycle transcript expression compared to the A549-EV cells. The geometric mean of two reference genes, H2AFY and ACTB was used. Asterisks (*) indicate p < 0.05. n = three per group for all panels. PDH, pyruvate dehydrogenase; PDP, Pyruvate dehydrogenase phosphatase; CS, Citrate synthase; ACO, Aconitase; IDH, Isocitrate dehydrogenase; OGDH, Oxoglutarate dehydrogenase; SUCLG1, Succinate-CoA ligase GDP/ADP-forming subunit alpha; SUCLG2, Succinate-CoA ligase GDP-forming subunit beta; SDH, Succinate dehydrogenase; FH, Fumarate hydratase; MDH, Malate dehydrogenase.