Figure 4.

The integrity and location of the Nup62 are altered during ZIKV infection. (A) Huh-7 cells mock-infected (Mock) or infected with ZIKV for 48 hrs, were incubated with a monoclonal anti-Nup62 antibody and analyzed by confocal microscopy. The anti-E protein antibody was used as control of infection. Nuclei were stained with DAPI. Representative images of three independent experiments are presented. (B) The graphs represent the results expressed as a percentage of Mock-normalized mean fluorescence intensity (MFI) arbitrary units and the error bars show the SD of three independent experiments, n = 30 per group. (C) Levels of Nup62 protein were analyzed by Western blot in whole-cell lysates from mock or ZIKV infected cells for 12, 24, and 48 hrs. The anti-NS3 antibody was used as a control of infection and anti-actin as a loading control. Representative Western blot assays from three independent experiments are presented. The graph represents Nup62 protein levels compared with β-actin. Protein amount values from control (mock-infected) were adjusted to a value of 1. Values for expression in infected cells (white) were then expressed as a number relative to the control. Data are means ± SEM of n = 3 independent experiments performed by duplicate.