Figure 9.

Transfection of NS2B3 proteins from DENV2 and ZIKV induces disruption of FG-rich sequence nucleoporins. (A) Huh-7 cells mock-transfected or transfected for 24 and 48 hrs with DENV2 or ZIKV active (WT) NS2B3 proteins were incubated with the Mab-414 and the anti-NS3 protein antibody respectively, and the integrity and subcellular localization of NS3 protein were analyzed by confocal microscopy. Nuclei were stained with Hoechst. Representative images of three independent experiments are presented. (B) The graphs represent the results expressed as a percentage of Mock-normalized mean fluorescence intensity (MFI) arbitrary units and the error bars show the SD of three independent experiments. **** p ≤ 0.0001, n = 30 per group.