Figure 4.

Isoflurane changes the calcium activity of arcuate nucleus neurons (A) 25-min-long in vivo recordings of arcuate nucleus neurons were performed in head-fixed mice, mice were then anaesthetised with isoflurane and the same neurons were recorded for another 25 min. (B) Time-lapse recordings of calcium (GCaMP6s) activity of 7 arcuate nucleus neurons in an awake adult C57Bl6 male. (C) Time-lapse recordings of calcium activity of the same 7 arcuate nucleus neurons in the same anaesthetised adult male.