Table 1.

Virus database for the transfection of hypothalamic neurons.

Serotype	Promoter	Transgene	Host Specie	Brain area	Neuronal Identity	References
9	EF1a	ChR2	mouse, rat	MS, rPOA, AHA	GnRH	(Campos & Herbison 2014) and unpublished data
9, 5, 2	EF1a, CAG	ChR2, GCaMP6s	mouse	ARN	GHRH	Unpublished data
9, 5, 2	EF1a, CAG	ChR2, GCaMP6s	mouse, rat	PVN, LH	CRH	(Pomrenze et al. 2015, Füzesi et al. 2016, Romanov et al. 2017)
9, 8, 5, 2	EF1a, CAG, Syn	ChR2, GCaMP6s GCaMP6m, hM3Dq	mouse	PVN,	TRH	(Krashes et al. 2014) and unpublished data
9	EF1a	ChR2, GCaMP6s, ArchR, HaloR hM3Dq, hM4Di	mouse	RP3V, ARN	Kisspeptin	(Han et al. 2015, Clarkson et al. 2017)
5	CAG	hM4Di	mouse	ARN	POMC	(Atasoy et al. 2012)
1, 8	Syn	ChR2, ArchR, GCamp6s, hM4Di	mouse	ARN	AgRP	(Atasoy et al. 2012, Krashes et al. 2014, Betley et al. 2015)
1, DJ,9, 5	Syn, Ef1a,	GCamp6s, ChETA, hM3Dq, eYFP	mouse	LH, ARN	GABAergic	(Jennings et al. 2015, Resendez et al. 2016, Silva et al. 2019)
1	Syn	GCamp6s	mouse	VMH	Galanergic	(Viskaitis et al. 2017)
1	Syn	GCamp6s	mouse	VMH	Estrogen receptor 1	(Remedios et al. 2017)

Brain area abbreviations: AHA, anterior hypothalamus; ARN, arcuate nucleus; LH, lateral hypothalamus; MS, medial septum; PVN, paraventricular nucleus; RP3V, rostral periventricular region of the third ventricle; rPOA, rostral preoptic area; VMH, ventro-medial hypothalamus.

Neuronal identity abbreviations: AgRP, Agouti-related peptide; CRH, corticotropin-releasing hormone; GABA: gamma-aminobutyric acid; GHRH, growth hormone-releasing hormone; GnRH, gonadotropin-releasing hormone; POMC, pro-opiomelanocortin; TRH, thyrotropin-releasing hormone.