Figure 7.

Inhibition of SIRT1-induced inflammation is partially reduced by P65 knockdown in PBMCs. (A–H) PBMCs were transfected with P65 siRNA or control siRNA and then the knockdown and control cells were treated with Ex527 (40 μM) or DMSO. The expression levels of TNF-α (A), IL-6 (B), IL-8 (C) and MCP1 (D) were examined by qRT-PCR (n = 3). (E–H) TNF-α (E), IL-6 (F), IL-8 (G) and MCP1 (H) concentrations in the culture supernatants were analyzed by ELISA (n = 3). (I) HEK-293T cells were transiently transfected with P65 siRNA or control siRNA. Ex527 and NF-κΒ promoter vectors were added 24 h before harvesting the cells, then transcriptional activities of NF-κB were analyzed by luciferase reporter assay (n = 3). Data represent means ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001.