This work is licensed under a Figure 4.
Gene expression of enzymes of the steroid hormone metabolism in the SGBS cell model during adipogenesis. The absolute mRNA expression of (A) the steroidogenic acute regulatory protein (StAR), (B) the cholesterol side-chain cleavage enzyme P450scc (CYP11A1), (C) 11beta-hydroxysteroid dehydrogenase 1 (HSD11B1), (D) the cytochrome P450 aromatase (CYP19) and (E) the aldo-keto reductase family 1 member C3 AKR1C3 (HSD17B5) normalized to 103 molecules of TBP as housekeeping gene was evaluated in SGBS cells during adipogenic differentiation under normal conditions (left). Additionally, AD stimulation is described in relation to the corresponding AD-free control level indicated as dotted line (right) (n = 4; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001 for normal adipogenesis in comparison to day 0 and # P ≤ 0.05; ## P ≤ 0.01; ### P ≤ 0.001 for comparing unexposed and AD-exposed cells).