This work is licensed under a Figure 5.
Influence of androstenedione on estrogen receptors in the SGBS cell model. The absolute mRNA expression of (A) estrogen receptor alpha (ERα) and (B) beta (ERβ) as well as (C) the G protein-coupled estrogen receptor 1 (GPER), (D) the androgen receptor (AR) and (E) the glucocorticoid receptor (GR) normalized to 103 molecules of TBP as housekeeping gene was evaluated in SGBS cells during adipogenic differentiation under normal conditions (left). Additionally, AD stimulation is described in relation to the corresponding AD-free control level indicated as dotted line (right) (n = 4; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001 for normal adipogenesis in comparison to day 0 and # P ≤ 0.05; ## P ≤ 0.01; ### P ≤ 0.001 for comparing unexposed and AD-exposed cells).