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. 2020 Jun 3;9(7):587–598. doi: 10.1530/EC-19-0549

Table 1.

Primers for quantitative RT-PCR.

Gene Accession number
Forward primer Reverse primer Tm (°C) Amplicon (bp)
AR NM_000044 AATTCCTGTGCATGAAAGCCA AGCTCTCTCGCAATAGGCTG 58 211
CYP19 NM_000103 ATGTGGACGTGTTGACCCTTCT AGGAGAGCTTGCCATGCATCAA 60 133
CYP11A1 NM_000781 CTCAGTCCTGGTCAAAGGCT CTTCTCCCTGTAAATCGGGC 63 250
ERα NM_000125 CAATGACTATGCTTCAGGCTAC CCACCTTTCATCATTCCCAC 60 198
ERβ NM_001437 AGCCACCATGAATATCCAGCCA TGGCCACAACACATTTGGGCTT 60 129
GLUT4 NM_001042 ACTGGCCATTGTTATCGGCA GTCAGGCGCTTCAGACTCTT 60 213
GPER NM_001505 ATGACCATCCCCGACCTGTA GAGGAAGAAGACGCTGCTGT 60 174
GR NM_000176 ACTGCTTCTCTCTTCAGTTC GATTTTCAACCACTTCATGC 60 168
HSD3B NM_000198 GGAAGCCAAGCAGAAAACCG GCCCCTGTTGCCTTCTGTAT 58 172
HSD11B1 NM_005525 CTCTACAGAAGGTGGTATCC AATGAGCATGTCTAGTCCTC 61 142
HSD17B5 NM_003739 TGAGGAGAAGCAGCAGCAAA GGCGGAACCCAGCTTCTATT 60 190
PGR NM_001202474 ATTCACTTTTTCACCAGGTC AACCTGGCAATGATTTAGAC 60 180
StAR NM_000349 GGCCTTGGGCATCCTTAG TCCACCACGACCTCCAG 60 131
TBP NM_003194 TGTGCTCACCCACCAACAAT AGTCGTCTTCCTGAATCCCT 60 199

AR, androgen receptor; CYP11A1, cholesterol side-chain cleavage enzyme P450scc; CYP19, cytochrome P450 aromatase; ERα and ERβ, estrogen receptor alpha and beta; GLUT4, glucose transporter 4; GPER, G protein-coupled estrogen receptor 1; GR, glucocorticoid receptor; HSD11B1, 11beta-hydroxysteroid dehydrogenase 1; HSD17b5, 17beta-hydroxy steroid dehydrogenase 5; HSD3B, 3beta-hydroxysteroid dehydrogenase; PGR, progesterone receptor; TBP, TATA-box-binding protein.