Table 1.
Gene | Accession number |
Forward primer | Reverse primer | Tm (°C) | Amplicon (bp) |
---|---|---|---|---|---|
AR | NM_000044 | AATTCCTGTGCATGAAAGCCA | AGCTCTCTCGCAATAGGCTG | 58 | 211 |
CYP19 | NM_000103 | ATGTGGACGTGTTGACCCTTCT | AGGAGAGCTTGCCATGCATCAA | 60 | 133 |
CYP11A1 | NM_000781 | CTCAGTCCTGGTCAAAGGCT | CTTCTCCCTGTAAATCGGGC | 63 | 250 |
ERα | NM_000125 | CAATGACTATGCTTCAGGCTAC | CCACCTTTCATCATTCCCAC | 60 | 198 |
ERβ | NM_001437 | AGCCACCATGAATATCCAGCCA | TGGCCACAACACATTTGGGCTT | 60 | 129 |
GLUT4 | NM_001042 | ACTGGCCATTGTTATCGGCA | GTCAGGCGCTTCAGACTCTT | 60 | 213 |
GPER | NM_001505 | ATGACCATCCCCGACCTGTA | GAGGAAGAAGACGCTGCTGT | 60 | 174 |
GR | NM_000176 | ACTGCTTCTCTCTTCAGTTC | GATTTTCAACCACTTCATGC | 60 | 168 |
HSD3B | NM_000198 | GGAAGCCAAGCAGAAAACCG | GCCCCTGTTGCCTTCTGTAT | 58 | 172 |
HSD11B1 | NM_005525 | CTCTACAGAAGGTGGTATCC | AATGAGCATGTCTAGTCCTC | 61 | 142 |
HSD17B5 | NM_003739 | TGAGGAGAAGCAGCAGCAAA | GGCGGAACCCAGCTTCTATT | 60 | 190 |
PGR | NM_001202474 | ATTCACTTTTTCACCAGGTC | AACCTGGCAATGATTTAGAC | 60 | 180 |
StAR | NM_000349 | GGCCTTGGGCATCCTTAG | TCCACCACGACCTCCAG | 60 | 131 |
TBP | NM_003194 | TGTGCTCACCCACCAACAAT | AGTCGTCTTCCTGAATCCCT | 60 | 199 |
AR, androgen receptor; CYP11A1, cholesterol side-chain cleavage enzyme P450scc; CYP19, cytochrome P450 aromatase; ERα and ERβ, estrogen receptor alpha and beta; GLUT4, glucose transporter 4; GPER, G protein-coupled estrogen receptor 1; GR, glucocorticoid receptor; HSD11B1, 11beta-hydroxysteroid dehydrogenase 1; HSD17b5, 17beta-hydroxy steroid dehydrogenase 5; HSD3B, 3beta-hydroxysteroid dehydrogenase; PGR, progesterone receptor; TBP, TATA-box-binding protein.