Table 2.

Steroid hormone analysis performed by LC-MS/MS.

Hormone	Normal adipogenesis (µmol/L)			+AD (µmol/L) day 8
	day 0	day 4	day 8
Aldosterone	0.005±0.002	0.032±0.007a	0.016±0.010	0.026±0.007
17alpha-hydroxyprogesterone	0.015±0.010	0.112±0.109	0.014±0.012	0.029±0.028
Androstenedione	4.957±1.260	13.918±2.968a	3.567±0.994	67.073±33.092
Androsterone	1.014±0.272	3.970±1.032a	0.847±0.219	158.753±13.614f
Corticosterone	0.005±0.003	0.291±0.037c	0.217±0.013c	0.219±0.023
Cortisol	0.301±0.155	34.853±1.926c	28.813±1.251c	28.813±1.585
Cortisone	0.080±0.019	2.112±0.137b	3.990±0.610c	8.770±0.659f
11-Deoxycorticosterone	0.013±0.013	0.578±0.366	0.091±0.068	0.013±0.008
11-Deoxycortisol	0.002±0.001	0.054±0.013b	0.015±0.002	0.054±0.006f
DHEA	0.080±0.080	0.160±0.090	0.861±0.428	0.033±0.021
Dihydrotestosterone	0.058±0.013	0.122±0.020a	0.056±0.010	6.689±1.106f
Estradiol	0.030±0.004	0.113±0.040	0.024±0.001	0.047±0.011
Estrone	0.760±0.701	0.176±0.049	0.099±0.036	0.046±0.011
Etiocholanolone	0.938±0.271	3.918±1.030a	0.832±0.219	158.673±13.619f
Progesterone	0.475±0.350	2.468±1.429	1.092±0.450	0.519±0.251
Testosterone	1.747±0.281	4.142±0.770a	1.260±0.270	99.833±21.772e

Data are presented as mean±s.e.m. (n = 5; ^aP ≤ 0.05; ^bP ≤ 0.01; ^cP ≤ 0.001 for normal adipogenesis in comparison to day 0 and ^dP ≤ 0.05; ^eP ≤ 0.01; ^fP ≤ 0.001 for comparing unexposed and androstenedione (AD)-exposed cells at day 8).