Figure 5.

PGC1A and leptin control HIF1A expression. (A) PGC1A mRNA levels were overexpressed by adenovirus infection (48 h) of MCF-7 cells. Whole-cell lysates were subjected to SDS-PAGE, blotted, and probed with anti-HIF1A, as indicated. β-actin (ACTB) was used as the loading control. (B) Relative expression of HIF1A mRNA was examined by real-time RT-PCR in PGC1A-infected MCF-7 cells. RPLP0 was used as the internal loading control. (C) After 48 h of incubation of MCF7 with different concentrations of leptin, whole-cell lysates were subjected to SDS-PAGE, blotted, and probed with anti-HIF1A. β-actin (ACTB) was used as the loading control. (D) Relative expression of HIF1A mRNA was examined by real-time RT-PCR in MCF-7 cells (48 h) treated with different concentrations of leptin. RPLP0 was used as the internal loading control. Data shown represent the means ± s.d. **P < 0.01.