Figure 6.

Effect of leptin on aromatase mRNA expression in control and Sam68-overexpressed GCs from healthy donors. GCs were transfected with Sam68 plasmid and pcDNA3 as control, as described in Materials and Methods, and incubated in absence or presence of leptin 10 nM for 48 h. Aromatase gene expression was analyzed by qRT-PCR, normalized against RPS-7. Data represent the mean ± s.d. value of triplicate samples in three independent experiments. Paired t-test was performed to examine the difference in mRNA level of aromatase, and statistical significance was considered when P value was < 0.05. * indicate significant differences from the control pcDNA3; # indicate significant differences from the control pcDNA3 with leptin and ^ indicate significant differences from the Sam68 plasmid, according to Bonferroni’s multiple comparison post hoc test.