Table 1. Information about chemical analysis of water and brain samples by liquidchromatography with mass spectrometry.
| TSQ Quantiva | Q-Exactive | ||||||
|---|---|---|---|---|---|---|---|
| Elution gradient for liquid chromatography | Elution gradient for liquid chromatography | ||||||
| Time [min] |
A [%] |
B [%] |
Flow [µL/min] |
Time [min] |
A [%] |
B [%] |
Flow [µL/min] |
| 0 | 100 | 0 | 300 | 0 | 100 | 0 | 350 |
| 1 | 100 | 0 | 300 | 1 | 100 | 0 | 350 |
| 5 | 60 | 40 | 350 | 3 | 50 | 50 | 350 |
| 7 | 20 | 80 | 400 | 4 | 20 | 80 | 450 |
| 8 | 0 | 100 | 400 | 5 | 0 | 100 | 450 |
| 8.01 | 100 | 0 | 300 | 6 | 0 | 100 | 450 |
| 10 | 100 | 0 | 300 | 6.05 | 100 | 0 | 350 |
| 7 | 100 | 0 | 350 | ||||
Notes.
A–mobile phase ultra-pure water + 0.1% formic acid; B –mobile phase acetonitrile + 0.1% formic acid.