Figure 2.
(a) Conjugation scheme of anti-CD4PEG-SH to NG. (b) Flow cytometry dot plots showing CD4 surface receptor staining intensity (on x-axis) and DiO intensity (y-axis). Co-cultured mT-ALL cells were incubated with anti-CD4PEG-PDS + NG(DiO) or anti-CD4PEG/NG(DiO) at 4 °C for 30 minutes (upper panel) or 37 °C for 3 hours (lower panel) at a concentration of 50 μg/mL of NG. CD4low cells are shown in red, CD4high cells are shown in blue color. (c) Bar plot shows the DiO mean fluorescence intensity of CD4low and CD4high mT-ALL cells. Error bars indicative of three replicates (S.D.)