Table 1.

Spectroscopic properties of FROG/B and its mutants

Name	Mutations (comparison with avGFP)	State	Absorption peak (nm)	FG/FB
PreFROG/B	K26R/S30R/Y39N/F64L/N105T/D150V/M153T/V163A/I171V/S175G/T203V/S205V/A206K/V224R/H231L	Ox	395	2.1
		Red	387	0.65
FROG/B	K26R/S30R/Y39N/F64L/Q69A/N105T/D150V/M153/V163A/I171V/S175G/T203V/S205V/A206K/V224R/H231L	Ox	399	2.3
		Red	389	0.5
PreFROG/BD150V	K26R/S30R/Y39N/F64L/Q69A/N105T/M153T/V163A/I171V/S175G/T203V/S205V/A206K/V224R/H231L	Ox	386	0.33
		Red	386	0.30
PreFROG/BH148G	K26R/S30R/Y39N/F64L/Q69A/N105T/H148G/D150V/M153/V163A/I171V/S175G/T203V/S205V/A206K/V224R/H231L	Ox	401	3.7
		Red	400	2.3