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. 2020 Jun 22;117(27):15632–15641. doi: 10.1073/pnas.1916343117

Fig. 7.

Fig. 7.

The unloaded velocity of KIF3AC is predicted by the force dependence of KIF3AA and KIF3CC velocities and recapitulated by MT gliding by mixtures of KIF3AA and KIF3CC. (A) Plot of MT velocity in a gliding filament assay versus percentage of KIF3CC observed for mixtures of KIF3AA and KIF3CC (red triangles) ± SD. The gliding velocity observed for KIF3AC alone is shown (unfilled blue circle) ± SD. The solid cyan squares represent the hypothetical predicted MT gliding velocity of any mixture of KIF3AA and KIF3CC based on the measured force dependence of the KIF3AA and KIF3CC homodimers in single-bead optical trapping assays (SI Appendix, Eq. S5), and the solid black circles show the predicted line multiplied by 1.3 to better align with the data. (B) Plot of velocity versus force with overlaid Bell equation fits for KIF3AA (blue) and KIF3CC (green). The red curve represents the fit of Eq. 1 to the KIF3AC data and the horizontal line (orange, dashed) intersects with the KIF3AC curve at zero force and crosses the KIF3AA and KIF3CC curves at ∼ ΔFA int and ΔFC int within the corresponding error, respectively. Shaded areas represent the 95% CI of the fits. (C) Parameters derived from the fit of the force dependence of velocity data for KIF3AC, KIF3AA, and KIF3CC.