Table 3.

Measured velocity (Vel) and run length (RL) of KIF3 motors

	1-pN Assisting		Unloaded		1-pN Hindering
Motor	Vel, nm/s	RL, nm	Vel, nm/s	RL, nm	Vel, nm/s	RL, nm
KIF3AA	190 ± 85	93 ± 68	240 ± 76	980 ± 910	140 ± 64	73 ± 62
KIF3CC	41 ± 22	54 ± 54	7.5 ± 6.3	570 ± 470	4.8 ± 16	25 ± 48
KIF3AC	170 ± 110	100 ± 57	170 ± 67	1300 ± 1700	68 ± 50	43 ± 48

Table of measured run lengths and velocities under 1-pN assisting load, unloaded, and 1-pN hindering load conditions at 1 mM MgATP. Displayed values are means ± SD. Unloaded values are included from previous Qdot single-molecule studies at 1 mM MgATP (14).