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. 2020 Jul 8;12:5561–5574. doi: 10.2147/CMAR.S243675

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© 2020 Hao et al.

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MiR-577 was negatively regulated by circ_0016760 in NSCLC cells. (A) The binding sites of circ_0016760 in miR-577 were predicted with the starBase 3.0. (B and C) The luciferase activities of circ_0016760-WT and circ_0016760-MUT in A549 and H1299 cells transfected with miR-577 or miR-NC were evaluated via dual-luciferase reporter assay. (D) After RNA pull-down assay, the enrichment of circ_0016760 was assessed with qRT-PCR. (E) QRT-PCR was utilized to analyze the expression of miR-577 in NSCLC tissues and neighboring normal tissues. (F) The correlation between circ_0016760 and miR-577 in NSCLC tissues was analyzed via Pearson’s correlation analysis. (G) QRT-PCR was used to assess the expression of miR-577 in A549, H1299, and 16HBE cells. (H) Influence of circ_0016760 repression on the expression of miR-577 of A549 and H1299 cells was explored via qRT-PCR. *P < 0.05.