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. 2020 Jul 8;12:5561–5574. doi: 10.2147/CMAR.S243675

Figure 5.

Figure 5

MiR-577 targeted ZBTB7A in NSCLC cells. (A) The binding sites between ZBTB7A and miR-577 were predicted with the starBase 3.0. (B and C) Dual-luciferase reporter assay was carried out to evaluate the luciferase activities of ZBTB7A-WT and ZBTB7A-MUT in A549 and H1299 cells transfected with miR-577 or miR-NC. (D and E) After RIP assay, the abundance of miR-577 and ZBTB7A was assessed via qRT-PCR. (F–I) The mRNA and protein levels of ZBTB7A in NSCLC tissues and neighboring normal tissues, as well as NSCLC cells (A549 and H1299 cells) and the 16HBE cells, were detected via qRT-PCR or Western blot analysis. (J) Pearson’s correlation analysis was performed to analyze the correlation between ZBTB7A and miR-577 in NSCLC tissues. (K and L) QRT-PCR or Western blot analysis was executed for analysis the levels of ZBTB7A mRNA and protein in A549 and H1299 cells transfected with anti-miR-577 or anti-NC. *P < 0.05.