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. 2020 Jul 8;12:5561–5574. doi: 10.2147/CMAR.S243675

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© 2020 Hao et al.

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miR-577 played its roles through ZBTB7A in NSCLC cells. (A) Western blot analysis was executed for the evaluation of the protein level of ZBTB7A in A549 and H1299 cells transfected with ZBTB7A or vector. (B–E) A549 and H1299 cells were transfected with miR-NC, miR-577, miR-577+vector, or miR-577+ZBTB7A. (B) The colony formation activity of A549 and H1299 cells was analyzed via colony formation assay. (C and D) Transwell assay was employed to determine the migration and invasion of A549 and H1299 cells. (E) ECAR assay was utilized to assess the ECAR of A549 and H1299 cells. *P < 0.05.