Figure 4.

Exogenous Nucleosides Improve Survival and the Ability of Human PSCs to Re-enter the Cell Cycle Post Plating

(A) Time-lapse data tracing the growth of individual MIFF1 cells that reach the first cell division plotted as lineage trees. A random sample of 20 cells (full set of 75 cells shown in Figure S4) grown without exogenous nucleosides (top, −NUC) and in the presence of exogenous nucleosides (bottom, +NUC). Where the trees fork indicates a cell division, red crosses show cell death, and surviving cells at the end of the time lapse are noted with a green triangle. Time is displayed on the y axis.

(B–D) Summary data of the lineage tree analysis (B). Histogram of the distribution colony sizes at the end of the time-lapse experiment (C). Schematic illustrating the scoring method used in (D). After each division, the fate of the daughter cells was recorded: both daughter cells surviving (SS), one daughter cell survives and one dies (SD), or both daughter cells die (DD). (D) Individual bar charts show the frequency of SS, SD and DD daughter cell fates following the first, second and third divisions (left to right) after plating (D). Cell culture media without exogenous nucleosides (black) and grown in the presence of exogenous nucleosides (red) conditions are shown.