Figure 2.

ME Cells Generate NS and Differentiate In Vitro to Neuro/Oligo Progenitors

(A) Explanation of NS culture and fixation schedule.

(B and D) Generation of NS from (B) isolated ME compared with (D) SVZ cells. Expended ME-NS express markers of neuronal (DCX), oligodendrocyte progenitors (NG2), and astrocytes (GFAP). Shown are bright field images of NS and immunostaining for neural markers.

(C and E) Temporal analysis of neural markers expressed by ME-NS (C) upon differentiation compared with (E) SVZ-NS and their quantitative analysis of (F) neuronal and (G) oligodendrocyte markers (G).

Scale bar, 50 μm.