Figure 3.

Increased Number of OPCs in the ME of EAE Mice

(A) Images show NG2/BrdU and DCX/BrdU staining in the DG, CC, and ME.

(B) Enhanced BrdU staining of ME in EAE mice compared with naive mice. Arrows indicate double-positive cells.

(C) Increased BrdU⁺ cells in EAE mice compared with naive mice (n = 5 per group).

(D) Macrophages (MAC2⁺) are not localized where OPCs are scored.

(E) NG2⁺ cells are distinctive from CD45⁺ cells.

(F and G) (F) Images of BrdU⁺ NG2⁺, BrdU⁺ RIP⁺ (arrowheads), and BrdU⁺ NG2⁺ RIP⁺ cells (arrows) in the ME of EAE mice and (G) their quantitative analysis (n = 4 per group).

(H) Immunostaining for DCX or β-tubulin with BrdU in the ME.

Scale bar, 50 μm. (C) ^∗p < 0.01, (G) ^∗p = 0.02.