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. 2020 May 14;14(6):1076–1092. doi: 10.1016/j.stemcr.2020.04.005

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Expression of TSP1 by ME- and SVZ-NSCs

(A and B) (A) Immunostaining for GFAP and TSP1 after the indicated time points of differentiation and (B) quantitative analysis of TSP1 fluorescence intensity (n = 6).

(C) Images of immunostaining for TSP1 in SVZ and ME.

(D) Quantitative analysis of TSP1 fluorescence intensity in SVZ and ME (n = 4 per group). ^∗p = 0.003, ^∗∗p = p < 0.001.

(E) Tsp1 relative expression in the ME and SVZ. ^∗p = 0.036.

(F) Orthogonal views of GFAP and TSP1 in ME and SVZ in naive mice compared with EAE mice. Low-power images of TSP1 are shown in Figure S4C. Scale bar, 50 μm.