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. 2020 May 14;14(6):1076–1092. doi: 10.1016/j.stemcr.2020.04.005

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Effect of TSP1 on Differentiation of ME-NSCs Compared with SVZ-NSCs

(A) Explanation of NS culture, differentiation in presence of TSP1, and fixation schedule.

(B) Staining for DCX and NG2 in ME- and SVZ-NSCs cultures treated with TSP1 or BSA. NSCs cultured in differentiation medium served as control. Shown are representative images out of three repeats.

(C and D) Quantitative analysis of (C) SVZ-NSC and (D) ME-NSC differentiation to DCX⁺ cells and NG2⁺ cells in the presence of TSP1 (mean ± SD). Scale bar, 50 μm. ^∗p ≤ 0.004, ^∗∗p ≤ 0.005.