Figure 6.
Effect of TSP1 Antagonist on Differentiation of ME-NSCs Compared with SVZ-NSCs
(A) Explanation of NS culture, differentiation in the presence of TSP1 antagonist or control peptide and fixation schedule.
(B) Staining for DCX and NG2 in ME- and SVZ-NSCs cultures treated with TSP1 antagonist (LSKL) or control peptide (SLLK) (50 μg/mL).
(C and D) Quantitative analysis of (C) SVZ-NSCs and (D) ME-NSCs differentiation to DCX+ and NG2+ cells in the presence of TSP1 antagonist (mean ± SD). Scale bar, 50 μm. ∗p ≤ 0.004, ∗∗p ≤ 0.015.