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. 2020 May 14;14(6):1076–1092. doi: 10.1016/j.stemcr.2020.04.005

Figure 7.

Figure 7

ME-Derived OLs Express MBP in Shi Mice

Coronal brain sections processed 3 weeks after injection of GFP-labeled NS into the LV.

(A) Images show GFP+ ME-NS (upper panel) or GFP+ SVZ-NS.

(B) Images show transplanted GFP+ ME-NSCs in the ME (upper panel) and SVZ (lower panel) expressing MBP (left column) or DCX (middle column). Upper images in the right column show higher magnification of GFP+ MBP+ in the CC. Scale bars, 20 μm and 10 μm (inset). The lower image is an orthogonal view showing localization of MBP with GFP+ ME-NSCs. Scale bar, 10 μm.

(C) Images show GFP+ SVZ-NSCs in the CC (upper panel) and adjacent LV (lower panel). Shown are immunostaining for MBP (left column) and DCX (middle column). Upper images in the right column show higher magnification of GFP+ DCX+ in the SVZ. Scale bars, 20 μm and 10 μm (inset). The lower image is orthogonal view showing localization of DCX with GFP+ SVZ-NSCs. Scale bar, 10 μm.

(D) Quantitative analysis of GFP+ cells co-express MBP or DCX in Shi mice transplanted with ME-NSCs compared with SVZ-NSCs (n = 5 per group). Scale bars, 50 μm (A–C). ∗p < 0.001.