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. 2020 Jun 9;14(6):1093–1106. doi: 10.1016/j.stemcr.2020.05.012

Figure 1.

Figure 1

VCAM1 Labels Adult NSCs in the SGZ of the Hippocampus

(A) Representative images illustrate isHCR staining (red) of VCAM1 in brain sections from the adult mice hippocampal DG (left). A high-magnification image of the box (left) is shown on the right. Nuclei were stained with Hoechst (blue).

(B and C) Representative images illustrate co-staining of VCAM1 (red) with NES, GFAP, or SOX2 (green or gray) (B), but not with NEUN or OLIG2 (green or gray) (C), in brain sections of adult WT mice by isHCR method.

(D) Representative images illustrate co-staining of VCAM1 (red) with GFAP, SOX2, and NES (green or gray) from acutely isolated single cells of DG in adult WT mice.

(E) Quantification of the proportion of VCAM1+ cells in NES+ GFAP+ cells, SOX2+ NES+ cells, and SOX2+ GFAP+ cells from acute staining of adult WT mice.

Nuclei were stained with Hoechst (blue). Scale bars, 250 μm (A, left), 10 μm (A, right), and 7.5 μm (B–D). Data represent mean ± SEM. (D–E) At least 1,000 cells were counted for each group.