Figure 3.

VCAM1 Is Preferentially Expressed in qNSCs in the Adult DG

(A) Schematic outline illustrates the experiment procedure of aNSCs/qNSCs harvested from the DG of adult mice in vitro.

(B) Representative images show neurospheres of aNSCs and qNSCs in the adult DG. Arrows indicate neurospheres.

(C and D) Expression of Ki67(C) and Vcam1 (D) mRNA in aNSCs versus qNSCs at the checking time revealed by qPCR.

(E) Representative staining images for neurosphere from aNSC and qNSC cultures (VCAM1 in red, GFAP in green, and NES in gray).

(F) Experimental schemes depict the short-term (left) BrdU pulse injections in adult mice infected by VP lentivirus.

(G) Representative images show Ki67 or BrdU staining (red) and GFP+ (green) cells in the SGZ of adult DG. Yellow arrows indicate GFP+ cells only, and white arrows indicate Ki67+ GFP+ cells.

(H and I) Quantification of the proportion of Ki67+ (H) and short-term BrdU+ (I) cells in total GFP+ cells through the SGZ of the entire DG.

Nuclei were stained with Hoechst (blue). Scale bars: (B) 100 μm, (E) 25 μm (top) and 7.5 μm (bottom), and (G) 25 μm. Data represent mean ± SEM. (C and D) Twelve repeats for each group; (H) 4 mice/147 GFP+ cells; (I) 3 mice/100 GFP+ cells; Student's t test for (C) and (D). ^∗∗∗∗p < 0.0001.