Table 1. List of materials used in this study.
| Name | Company | Catalogue
Number |
||
|---|---|---|---|---|
|
CytoTune-iPS 2.0 Sendai
Reprogramming Kit |
Thermo Fisher | A16517 | ||
| Essential 8™ medium | Thermo Fisher | A1517001 | ||
| NUNC™ plates | Thermo Fisher | 140675 | ||
| Geltrex™ | Thermo Fisher | A1413302 | ||
| Versene (EDTA) solution | Lonza | BE17-711E | ||
| Hank's Balanced Salt Solution (HBSS) | Thermo Fisher | 14170-161 | ||
|
Dulbecco’s Modified Eagle’s Medium/
Nutrient Mixture F-12 Ham |
Sigma Aldrich | D6421 | ||
| GlutaMAX™ | Thermo Fisher | 35050-061 | ||
| N-2 (100X) | Thermo Fisher | 17502-048 | ||
| Neurobasal® medium | Thermo Fisher | 21103-049 | ||
| B-27 minus vitamin A (50X) | Thermo Fisher | 12587-010 | ||
| LDN193189 | Sigma Aldrich | SML0559 | ||
| SB431542 | Sigma Aldrich | S4317 | ||
| XAV939 | Sigma Aldrich | X3004 | ||
| Cyclopamine | LC Laboratories | C-8700 | ||
| Y-27632 | Sigma Aldrich | Y0503 | ||
| DNeasy Blood & Tissue Kit | QIAGEN | 69504 | ||
| Taq DNA Polymerase | QIAGEN | 201203 | ||
| HhaI digestion enzyme | Thermo Fisher | ER1851 | ||
| TRIzol® reagent | Thermo Fisher | 15596026 | ||
|
SuperScript® III First-Strand
Synthesis System |
Thermo Fisher | 18080051 | ||
| HOT FIREPol® EvaGreen® qPCR Mix | Solis Biodyne | 08-24-00001 | ||
| Name | Concentration | Host/Clonality | Company | Catalogue Number |
| Hoechst 33342 | 1:2000 | - | Thermo Fisher | H3570 |
| anti-human SOX2 | 1:1000 | Rabbit monoclonal | Millipore | AB5603 |
| anti-human TBR2 | 1:250 | Rabbit polyclonal | Abcam | AB23345 |
| anti-human ApoE | 1:200 | Goat polyclonal | Millipore | AB947 |
| anti-Rabbit Alexa Fluor 488 | 1:500 | Donkey polyclonal | Thermo Fisher | A21206 |
| anti-Goat Alexa Fluor 594 | 1:500 | Donkey polyclonal | Thermo Fisher | A11058 |