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. 2020 Jul 7;32(1):107862. doi: 10.1016/j.celrep.2020.107862

Figure 3.

Figure 3

Nog2 Diffuses from the Notochord to the Pancreatic Progenitor Domain

(A) Representative confocal image of a 18 hpf zebrafish embryo injected with the nog2E3:GFPnog2 construct. Of 20 embryos, 16 (80%) showed colocalization of GFPNog2 aggregates (green) with pancreatic progenitor cells, labeled with anti-Nkx6.1 antibody (white). Details of the colocalization can be observed in the inset (white box).

(B) Representative confocal image of a 18 hpf zebrafish embryo injected with nog2E3:GFP (control), in which the pattern of GFP expression (green) is restricted to the notochord. None of 18 analyzed embryos displayed colocalization of GFP aggregates with Nkx6.1-labeled cells. Embryos were counterstained with the nuclear marker DAPI (blue), and the endocrine progenitor domain is delimited by a yellow dashed line (see also Figure S2). Images represent the maximum-intensity z projection of several focal planes obtained in a Leica Sp5 confocal microscope using a 40× objective. Scale bars represent 30 μm.